ABSTRACT
INTRODUCTION: Traumatic spinal cord injury is one of the most disabling conditions occurring in man and thus stimulates a strong interest in its histopathological, biochemical, and functional changes, primarily as we search for preventive and therapeutic methods. PURPOSE: To develop an experimental model for transplantation of cells from the fetal rat central nervous system to the site of an injured spinal cord of an adult rat in which the transplanted cells survive and become integrated. This experimental model will facilitate investigations of factors that promote regeneration and functional recovery after spinal cord trauma. MATERIAL AND METHODS: Fifteen adult Wistar rats underwent laminectomy, and an spinal cord lesion was made with microdissection. Fetal spinal cord tissue was then transplanted to the site of the injury. The rats were monitored over a 48-hour period, and then their vertebral column was completely removed for histological analysis. RESULTS: In 60 percent of transplanted rats, the fetal tissue at the injured site remained viable in the site of the lesion
Subject(s)
Animals , Male , Female , Rats , Fetal Tissue Transplantation , Spinal Cord , Spinal Cord Injuries , Stem Cell Transplantation , Disease Models, Animal , Nerve Tissue , Rats, Wistar , Spinal CordABSTRACT
Dada a relevancia que o tromboembolismo pulmonar (TEP) vem adquirindo nos ultimos tempos, realizamos um estudo epidemiologico retrospectivo (caso-controle) embasado na analise de 1290 laudos de necropsias consecutivas realizadas em 1993 e 1994, no HCFMUSP, com posterior analise estatistica dos dados obtidos atraves dos programas Multlr e SPSS for Windows. Notamos que a incidencia de TEP foi de 12 por cento e que alguns fatores de risco como TVP (O.R.=13,98), trombose mural (O.R.=10,23), cirurgia ortopedica (O.R.=2,71), cirurgia neurologica (O.R.=4,16), doenca arterial obstrutiva periferica (O.R.=3,12) e ICC (O.R.=2,43) contribuem para o seu desenvolvimento. A insuficiencia hepatica apareceu como fator protetor (O.R.=0,77). Desta forma, achamos que a profilaxia nestes casos e necessaria para prevenir o TEP